细胞骨架/SiR700微管蛋白试剂盒/35 nmol SiR700微管蛋白和1µmol维拉帕米；weee><span-免疫在线蚂蚁淘旗下平台

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商品详细细胞骨架/SiR700微管蛋白试剂盒/35 nmol SiR700微管蛋白和1µmol维拉帕米；weee>

细胞骨架/SiR700微管蛋白试剂盒/35 nmol SiR700微管蛋白和1µmol维拉帕米；weee>

商品编号： CY-SC014

品牌： Cytoskeleton

市场价： ¥10300.00

美元价： 6180.00

产地：美国(厂家直采)

公司：

产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Details

SiR700-tubulin is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the microtubule-binding drug Docetaxel. SiR700-tubulin allows labeling of endogenous microtubules in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-tubulin are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700-tubulin can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-tubulin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filter sets. Probe quantity allows 35 – 140 staining experiments.*

Optical properties

λ_abs 689 nm

λ_Em 716 nm

ε_max 1.0·10⁵ mol^-1·cm^-1

MW 1327.6 g/mol

MF C₇₅H₈₆N₄O₁₆Si

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiment with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.

Fibro_1h_1700_nM_SiR700_C8_tubulin_Hoechst_washed-RESIZED

Fibroblast cells stained with SiR700-tubulin (red) (Cat. # CY-SC014) and Hoescht (blue).

About

For product Datasheets and MSDSs please click on the PDF links below.

Spirochrome Technical Tips and Ex/Em spectra in graphical form (PDF)

Citations

Featured Papers

"Fluorogenic probes for multi-color imaging in living cells." Lukinavicius G. et al. 2016. J. Am. Chem. Soc. Doi: 10.1021/jacs.6b04782.

“Fluorogenic probes for live-cell imaging of the cytoskeleton”; G. Lukinavičius, L.Reymond, E. D’Este, A. Masharina, F. Göttfert, H. Ta, A. Güther, M. Fournier, S. Rizzo, H. Waldmann, C. Blaukopf, C. Sommer, D. W. Gerlich, H.-D. Arndt, S. W. Hell & K. Johnsson; Nature Methods 11, 731–733, 2014.

“STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons”; E. D’Este, D. Kamin, F. Göttfert, A. El-Hady, S. W. Hell; Cell Reports , Volume 10 , Issue 8 , 1246 – 1251, 2015.

“A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins”; G. Lukinavičius, K. Umezawa, N. Olivier, A. Honigmann, G. Yang, T. Plass, V. Mueller, L. Reymond, I. R. Corrêa Jr, Z. Luo, C. Schultz, E. A. Lemke, P. Heppenstall, C. Eggeling, S. Manley & K. Johnsson; Nature Chemistry 5, 132–139, 2013.

“Dynamic actin filaments control the mechanical behavior of the human red blood cell membrane”; D. S. Gokhin, R. B. Nowak, J. A. Khoory, A. de la Piedra, I. C. Ghiran and V. M. Fowler; Mol. Biol. Cell; February 25, 2015.

“A cleavable cytolysin-neuropeptide Y bioconjugate enables specific drug delivery and demonstrates intracellular mode of action”; V. M. Ahrens, K. B. Kostelnik, R. Rennert, D. Böhme, S. Kalkhof, D. Kosel, L. Weber, M. von Bergen and A. G. Beck-Sickinger; J. Control. Release; 209:170-178, 2015.

“Red Si–rhodamine drug conjugates enable imaging in GFP cells”; E. Kim, K. S. Yang, R. J. Giedt and R. Weissleder; Chem. Commun., 50, 4504-4507, 2014.

“A marginal band of microtubules transports and organizes mitochondria in retinal bipolar synaptic terminals”; M. Graffe, D. Zenisek, and J. Taraska; J. Gen Physiol. Vol. 146 No.1: 109-117, 2015.

Application Notes

“A Bright Dye for Live-Cell STED Microscopy”; S. Pitsch, I. Köster.

品牌介绍

Cytoskeleton，Inc.成立于1993年。自成立以来，我们一直在不断扩大产品范围。Cytoskeleton，Inc.很高兴为药物筛选，信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于纯化蛋白的生产和易于使用的试剂盒，以研究生化和细胞过程。我们的试剂盒既可用于基础研究或小型筛查的少量样品，也可用于大型筛查的高通量规模除了我们现有的产品外，我们还提供产品系列中微管，微管蛋白，运动蛋白，小G蛋白效应物，GAP，GEF和其他几种蛋白的药物筛选服务。有关更多信息，请参见我们的药物筛选服务页面。如果您想从市场上购买到特定产品，请随时与我们联系。我们在这里为您提供帮助。由于我们的科学家在各自的专业领域都有多年的工作经验，因此我们能够提供高质量的产品。自1993年成立以来，我们以合理的价格生产优质的产品而闻名。

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