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当前位置： 首页 > 产品中心 > Small_molecule > 细胞骨架/泛素化控制珠/10个反应/CUB02珠
商品详细细胞骨架/泛素化控制珠/10个反应/CUB02珠
细胞骨架/泛素化控制珠/10个反应/CUB02珠
细胞骨架/泛素化控制珠/10个反应/CUB02珠
商品编号： CUB02-beads
品牌： Cytoskeleton
市场价： ¥1900.00
美元价： 1140.00
产地： 美国(厂家直采)
公司：
产品分类： 小分子
公司分类： Small_molecule
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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SigSeekGraphic2

As part of the Signal-Seeker™ product line, ubiquitination control beads (CUB02) have been developed as highly specific negative control beads for Cytoskeleton"s ubiquitination affinity bead (UBA01-Beads) products. These control beads are far superior to beads alone, because it has mutated versions of the UBDs covalently attached to the control bead matrix. This control is particularly important for ubiquitin IPs as the unmodified version of target proteins often bind non-specifically to all available ubiquitin enrichment affinity matrices. A comprehensive Signal-Seeker™ Ubiquitin Detection Kit (BK161), which includes ubiquitin control beads (CUB02), is available and is recommended for first time users.

Validation Data: Ubiquitination Control Beads White Paper

Each lot of affinity-bead is quality controlled  to provide high batch to batch consistency, see COA documents.

Validated Applications

Application 1: Utilization of IgG control beads

The activity of the Rho family of proteins, including Rac1, is known to be regulated, in part, through ubiquitination (also termed ubiquitylation) events that can lead to signaling pathway regulation via degradation and/or localization of the modified protein (Visvikis, O. et al. 2010. Biol. Cell 102: 377-389. Nethe, M., & Hordijik, P.2010. J. Cell Sci. 123: 4011-4018). In many cases, the GTP-bound active form of Rac1 is the preferred substrate for ubiquitination. For example, it has been shown that cells treated with the bacterial toxin CNF1 leads to constitutive activation of Rac1 and subsequent mono- and poly-ubiquitination (Pop, M. et al. 2004. J. Biol.Chem. 279: 35840-35848).
Using the Signal Seeker™ Ubiquitination Detection Kit (Cat # BK161) we examined the ubiquitination of endogenous Rac1 in 3T3 cells treated with CNF1 toxin (Cat # CN04) and found that both mono- and polyubiquitination of Rac1 could be detected from 300 μg of 3T3 cell lysate.
Importantly, note that the non-specific interaction of unmodified Rac1 occurs with both ubiquitination affinity beads (UBA01) as well as ubiquitination control beads (CUB02) (lanes 2-5); however, the mono- and poly-ubiquitinated Rac1 only binds to ubiquitination affinity beads (lane 3) and not to the ubiquitination control beads (lane 5).  This data shows the utility of having appropriate control beads to help users distinguish the unmodified target protein band that is often present when performing ubiquitin IPs, which often occurs with every available ubiquitination enrichment tool.  
CUB02

Figure 1: Swiss 3T3 cells were pre-treated with MG-132, then either untreated or treated with CNF1 (CN04) for 3 hours prior to lysis with BlastR buffer. The BK161 kit was utilized to perform the IP on 300 μg of lysate per condition. Input: 3 μg input untreated lysate, Ubiquitin Affinity beads (UBA01) plus untreated lysate, UBA01 plus CN04 treated lysate, ubiquitin control beads (CUB02) plus untreated lysate, and CUB02 plus CN04 treated lysate. Samples were analyzed for Rac1 ubiquitination using an anti-Rac1 antibody.

Amount:Each package contains enough ubiquitin control beads for 10 reactions.

 

For more information contact:  signalseeker@cytoskeleton.com

Associated Products:

Signal-Seeker™ Ubiquitination Detection Kit (Cat. # BK161)

Signal-Seeker™ Ubiquitin Affinity Beads (Cat.# UBA01-beads)

Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)

Signal-Seeker™: PTMtrue™ Ubiquitin Antibody (Cat.# AUB01)

About

    Citations

    For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here

    Faqs

    Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here

    If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com

    品牌介绍
    Cytoskeleton，Inc.成立于1993年。自成立以来，我们一直在不断扩大产品范围。Cytoskeleton，Inc.很高兴为药物筛选，信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于纯化蛋白的生产和易于使用的试剂盒，以研究生化和细胞过程。我们的试剂盒既可用于基础研究或小型筛查的少量样品，也可用于大型筛查的高通量规模除了我们现有的产品外，我们还提供产品系列中微管，微管蛋白，运动蛋白，小G蛋白效应物，GAP，GEF和其他几种蛋白的药物筛选服务。有关更多信息，请参见我们的药物筛选服务页面。如果您想从市场上购买到特定产品，请随时与我们联系。我们在这里为您提供帮助。由于我们的科学家在各自的专业领域都有多年的工作经验，因此我们能够提供高质量的产品。自1993年成立以来，我们以合理的价格生产优质的产品而闻名。
    公司介绍
    Cytoskeleton，Inc.成立于1993年。自成立以来，我们一直在不断扩大产品范围。Cytoskeleton，Inc.很高兴为药物筛选，信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于纯化蛋白的生产和易于使用的试剂盒，以研究生化和细胞过程。我们的试剂盒既可用于基础研究或小型筛查的少量样品，也可用于大型筛查的高通量规模除了我们现有的产品外，我们还提供产品系列中微管，微管蛋白，运动蛋白
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